Download the index At Stanford -- for Sherlock users who are using the HG19 genome, copy or point Machete at this directory “/scratch/PI/horence/gillian/HG19_reg_indels/IndelIndices/” Outside of Stanford - please email glhsieh@stanford.edu.
Supported Organisms: Human (hg18, hg19, hg38), Mouse (mm8, mm9, mm10), Rat (rn4, rn5, rn6), Frog (xenTro2, xenTro3), Zebrafish (danRer7), Drosophila (dm3), C elegans (ce6, ce10), S. Download genomes the easy way. Contribute to simonvh/genomepy development by creating an account on GitHub. (No maintenance) OpenGene, core libraries for NGS data analysis and bioinformatics in Julia - OpenGene/OpenGene.jl #Download your gene set of interest for hg19. For this example, I'll use the refGene table, #but you can choose other gene sets, such as the knownGene table from the "UCSC Genes" track. $rsync -a -P rsync://hgdownload.soe.ucsc.edu/goldenPath… A tool to identify ethnicity given a vcf file and to generate ethnic population-specific reference genomes - alexanderhsieh/ethref Go / Golang Bioinformatics Library. Contribute to pbenner/gonetics development by creating an account on GitHub.
To start we first need to download a chain file specific to the assembly conversion we the chain file wget -c http://hgdownload.cse.ucsc.edu/goldenPath/hg19/liftOver/ Download complete GTF files from Ensembl represent all gene/transcript MD5 checksums are provided for verifying file integrity after download. Additional files are also GDC.h38 GENCODE v22 GTF (used in RNA-Seq alignment and by HTSeq). gencode.v22.annotation.gtf.gz TCGA.hg19.June2011.gaf. [kaiwang@biocluster ~/]$ annotate_variation.pl -downdb -buildver hg19 This command downloads a few files and save them in the humandb/ annotation database, we recommend using the GTF file to generate the gene definition file. Welcome to the download center supported by NONCODE! This is an Open data distributed ID conversion file (format: NONCODE ID, Source, Source ID) Space Ranger provides pre-built human (hg19, GRCh38), mouse (mm10), Your FASTA and GTF files must be compatible with the open source In Ensembl, the recommended genome file to download is annotated as "primary assembly.
GFF format: http://genome.ucsc.edu/FAQ/FAQformat.html#format3 GTF format: http://genome.ucsc.edu/FAQ/FAQformat.html#format4 Example1 (write output to file) --- CrossMap.py gff hg19ToHg18.over.chain.gz test.hg19.gtf test.hg18.gtf Example2… tophat2 -p 2 \ -G "/fdb/igenomes/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" \ --transcriptome-index =transcriptome_data/known \ "/fdb/igenomes/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/genome" hg19.bowtie2_index/hg19_trans/hg19_known_ensemble_trans.* How to get: download from (http://bowtie-bio.sourceforge.net/bowtie2/index.shtml) or follow instructions provided by bowtie2. Download the index At Stanford -- for Sherlock users who are using the HG19 genome, copy or point Machete at this directory “/scratch/PI/horence/gillian/HG19_reg_indels/IndelIndices/” Outside of Stanford - please email glhsieh@stanford.edu. Annotate variant nomenclature. Contribute to jiwoongbio/Annomen development by creating an account on GitHub.
A tool to identify ethnicity given a vcf file and to generate ethnic population-specific reference genomes - alexanderhsieh/ethref
Set of helpful scripts. Contribute to michael-kotliar/scripts development by creating an account on GitHub. A large number of computational methods have been developed for analyzing differential gene expression in RNA-seq data. We describe a comprehensive evaluation of common methods using the SEQC benchmark dataset and Encode data. stringtie的输入BAM文件需要先进行sort samtools view -Su alns.sam | samtools sort - alns.sorted stringtie alns.sorted.bam -b stringtie_input_dir -e -G hg19.annotation.gtf -C cov_ref.gtf -p 7 -o stringtie.out.gtf # -B This switch enables the… Supported Organisms: Human (hg18, hg19, hg38), Mouse (mm8, mm9, mm10), Rat (rn4, rn5, rn6), Frog (xenTro2, xenTro3), Zebrafish (danRer7), Drosophila (dm3), C elegans (ce6, ce10), S. Download genomes the easy way. Contribute to simonvh/genomepy development by creating an account on GitHub. (No maintenance) OpenGene, core libraries for NGS data analysis and bioinformatics in Julia - OpenGene/OpenGene.jl #Download your gene set of interest for hg19. For this example, I'll use the refGene table, #but you can choose other gene sets, such as the knownGene table from the "UCSC Genes" track. $rsync -a -P rsync://hgdownload.soe.ucsc.edu/goldenPath…