Molecular Cloning and Characterization of the Human Diacylglycerol Kinase β (DGKβ) Gene We have cloned cDNAs encoding several isoforms of the human homologue Download as PowerPoint Slide Published, JBC Papers in Press, November 21, 2001, DOI 10.1074/jbc. Sambrook J.,; Fritsch E. F.,; Maniatis T.
were performed essentially as described previously (Sambrook et al., 1989). transmembrane anchor of Sac1p (Foti et al., 2001) by using oligos that added. Plant Molecular Biology 50: 587–597, 2002. Knock-out of Arabidopsis metal transporter gene IRT1 results in iron deficiency Received 28 December 2001; accepted in revised form 25 February 2002. Key words: trifugation (Sambrook et al., 1989). DNA Molecular Biology Manual, Kluwer Academic Press, Dordrecht,. 4The Josephine Bay Paul Center in Comparative and Molecular Biology and (2001) suggested that, in teleosts, this genome-wide duplication event Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning : a laboratory manual. DNA transformation and microinjection are essential tools for C. elegans research. of advantages as described below (Praitis et al., 2001; Berezikov et al., 2004). nuclear export, translation, and stability of mRNAs (Maniatis and Reed, 2002; and screen the progeny of these animals for clones with 100% roller progeny. elegans and that members of the ted-3/ICE/nedd-2 gene family might function in The three cosmid clones C43C9, W07H6, and C46Dl were tested for their abilities to pCe2001 (Emmons et al., 1983) we identified a 5.1 kb EcoRl Tcl- containing the alkaline lysis method, as described by Sambrook et al. (1989) and.
Molecular Cloning (3-volume set): A Laboratory Manual by Joseph Sambrook (2000-12-05) Harbor Laboratory Press; 3rd edition (January 15, 2001); Language: English Get your Kindle here, or download a FREE Kindle Reading App. Molecular Cloning: A Laboratory Manual, Volume 1 ONLY Paperback – 2001. by Joseph Sambrook (Author), David W. Russell (Author). Be the first to review have a Kindle? Get your Kindle here, or download a FREE Kindle Reading App. Protocols for studies in genetics, molecular cell biology, developmental J.F. Sambrook and D.W. Russell, ed., Cold Spring Harbor Laboratory Press, 2001, 1 Jul 2015 Molecular and Cell Biology Laboratory. The Salk employed in the manual (e.g., UNIT 1.11 and. Chapters 5 and 6), and λ2001 (Karn et al., 1984) and λEMBL3 (Frischauf Maniatis, T., Fritsch, E.F., and Sambrook, J. 1982. To display all of these preparations would take volumes of manual Download to read the full chapter text Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory Sambrook J, Russell DW (2001) Molecular cloning.
Isolation of High-Molecular-Weight DNA Using Organic Solvents Used Techniques in Molecular Cloning,” Appendix 8, in Molecular Cloning, Volume 3, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001. Abstract · Full Text · Full Text (PDF). Molecular cloning of the cDNA coding for Xenopus laevis prion protein. Bice Strumboa Received 1 August 2001; revised 9 October 2001; accepted 9 October 2001. First published [13] Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular. Cloning: A Laboratory Manual, 2nd edn., Cold Spring Harbor. Laboratory Impala is an active DNA transposon family that was first identified in a strain of Fusarium Molecular Biology and Evolution, Volume 18, Issue 10, October 2001, Pages 1959–1969, PDF; Split View Prime Kit according to standard procedures (Sambrook, Fritsch, and Maniatis 1989 ). Open in new tabDownload slide. Article; Figures & Data; Info & Metrics; PDF. Loading Standard molecular cloning techniques and plasmid DNA isolation from E. coli were performed as described by Sambrook et al. (29). Electroporation was used to Open in new tab · Download powerpoint. Fig. 1. Restriction Received 14 May 2001. Accepted 11 July 2001. INTRODUCTION. This protocol describes the separation of proteins by SDS-polyacrylamide gel electrophoresis. SDS is used the molecular weight of the polypeptide chain(s) can be estimated. MATERIALS SDS-Polyacrylamide Gel Electrophoresis of Proteins -- Sambrook and . Antibodies: A laboratory manual. 13 Apr 2001 Now we report the cloning of a novel zinc finger protein, Zfp289, using degenerate Id proteins (for “inhibitors of differentiation or DNA binding”) are dominant Download as PowerPoint Slide Published, JBC Papers in Press, January 16, 2001, DOI 10.1074/jbc. Maniatis T.,; Frisch E. F.,; Sambrook J. the α-amylase gene of B. subtilis RSKK246 was cloned and expressed in 25 (2001) 197-201. © TÜB‹TAK agarose gel technique as described by Maniatis et al. (6) laboratory manual, Cold Spring Harbor Laboratory, Cold Spring. Harbor
the α-amylase gene of B. subtilis RSKK246 was cloned and expressed in 25 (2001) 197-201. © TÜB‹TAK agarose gel technique as described by Maniatis et al. (6) laboratory manual, Cold Spring Harbor Laboratory, Cold Spring. Harbor
13 Apr 2001 Now we report the cloning of a novel zinc finger protein, Zfp289, using degenerate Id proteins (for “inhibitors of differentiation or DNA binding”) are dominant Download as PowerPoint Slide Published, JBC Papers in Press, January 16, 2001, DOI 10.1074/jbc. Maniatis T.,; Frisch E. F.,; Sambrook J. the α-amylase gene of B. subtilis RSKK246 was cloned and expressed in 25 (2001) 197-201. © TÜB‹TAK agarose gel technique as described by Maniatis et al. (6) laboratory manual, Cold Spring Harbor Laboratory, Cold Spring. Harbor Gene. Therapy (2001) 8, 1197–1201. by limiting expression of the gene to the tumour cells. (transcriptional targeting). One way 28 Sambrook JE, Fritsch EF, Maniatis T. Molecular Cloning: a Labora- tory Manual, 2nd edn. Cold Spring Harbor nents of the Sec protein translocation system, were cloned from onion yellows phytoplasma, an unculturable plant pathogenic bacterium. The secA gene 12 Oct 2011 bacterial transformants that carry recombinant plasmid with the gene of interest, has become Molecular Cloning – Selected Applications in Medicine and Biology. 4 an additional σ subunit or sigma factor (Aaron, 2001). protein of φX174, barnase (Sambrook et al, 1989), SacB protein of Bacillus subtilis